In Vitro Capacitation of Bull Spermatozoa: Role of Vitamin E
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چکیده
The aim of this work was to study the influence of vitamin E (2mM) on capacitation and acrosome reaction of crossbred cattle bull spermatozoa. Fresh semen suspended in TALP medium (pH 7.4) was divided into four equal fractions. All fractions; control (containing TALP + spermatozoa) and the experimental [treated / untreated with ferrous ascorbate (FeAA – 150 μM FeSO4 : 750 μM ascorbic acid); supplemented / un-supplemented with 2mM vitamin E] were incubated for 6 h at 37°C. These fractions were assessed for % hyperactivity, morphology, lipid peroxidation (LPO), % acrosome reaction and changes in protein contents during sperm capacitation. FeAA treatment non-significantly (P≥0.05) decreased % sperm hyperactivity, % acrosome reaction and protein contents, but, increased LPO level. However, supplementation of vitamin E to the FeAA treated / untreated fractions non-significantly (P ≥0.05) altered % hyperactivity, rate of acrosome reaction, protein content, but, significantly (P≤0.05) decreased LPO level of bull spermatozoa during capacitation. It is concluded that vitamin E protects the sperm against LPO, and, alters its functions, though not effectively.
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